Reporter

Part:BBa_K2765043:Design

Designed by: Fa He   Group: iGEM18_BIT-China   (2018-09-25)


Produce redox sensitive Green fluorescent protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 843
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1155


Design Notes

The roGFP2-orp1 is a fusion protein, and both of these proteins need to fold. In order not to affect their structure, we added Linker between them to make sure that their structure didn't go wrong


Source

We got the sequence from cornrll 2017,and synthesised it by ourselves.

References